6 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Eufaula | 1 | 12,169 | 12,873 | -1.15% | 204 | 59.52 | City |
| Clayton | 2 | 2,180 | 2,259 | -0.73% | 322 | 6.78 | Town |
| Clio | 3 | 1,188 | 1,218 | -0.5% | 118 | 10.07 | City |
| Louisville | 4 | 385 | 397 | -0.52% | 140 | 2.75 | Town |
| Bakerhill | 5 | 205 | 215 | -0.97% | 75 | 2.75 | Town |
| Blue Springs | 6 | 76 | 83 | -1.3% | 26 | 2.90 | Town |
Eufaula is the largest city in Barbour County, with a population of 12,169.
Blue Springs is the smallest city in Barbour County.
There are 6 cities in Barbour County, Alabama.