14 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Valley | 1 | 10,073 | 10,445 | -0.7% | 781 | 13 | City |
| Valley | 2 | 10,073 | 10,426 | -0.7% | 781 | 13 | City |
| Lanett | 3 | 6,646 | 6,968 | -0.87% | 1,065 | 6 | City |
| Lanett | 4 | 6,646 | 6,934 | -0.87% | 1,065 | 6 | City |
| Huguley | 5 | 3,140 | 2,819 | 1.29% | 380 | 8 | CDP |
| La Fayette | 6 | 2,586 | 2,682 | -0.65% | 292 | 9 | City |
| La Fayette | 7 | 2,586 | 2,671 | -0.65% | 292 | 9 | City |
| Rock Mills | 8 | 387 | 434 | -3.73% | 61 | 6 | CDP |
| Abanda | 9 | 371 | 192 | 3.34% | 124 | 3 | CDP |
| Fredonia | 10 | 240 | 226 | 0.84% | 20 | 12 | CDP |
| Five Points | 11 | 115 | 118 | -0.86% | 112 | 1 | Town |
| Five Points | 12 | 115 | 117 | -0.86% | 112 | 1 | Town |
| Standing Rock | 13 | 35 | 109 | -20.45% | 11 | 3 | CDP |
| Penton | 14 | 31 | 53 | -26.19% | 3 | 9 | CDP |
Valley is the largest city in Chambers County, with a population of 10,073.
Penton is the smallest city in Chambers County, with a population of 31.
There are 14 cities in Chambers County, Alabama.