8 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Muscle Shoals | 1 | 17,772 | 16,367 | 1.61% | 1,054 | 16.87 | City |
| Sheffield | 2 | 9,253 | 9,388 | -0.29% | 1,324 | 6.99 | City |
| Tuscumbia | 3 | 9,245 | 9,054 | 0.41% | 1,003 | 9.21 | City |
| Littleville | 4 | 1,045 | 1,045 | 0% | 210 | 4.97 | Town |
| Cherokee | 5 | 962 | 972 | -0.21% | 434 | 2.22 | Town |
| Leighton | 6 | 655 | 649 | 0.15% | 304 | 2.16 | Town |
| Hatton | 7 | 355 | 59 | 2.01% | 266 | 1.33 | CDP |
| Belgreen | 8 | 5 | 27 | -64.29% | 4 | 1.23 | CDP |
Muscle Shoals is the largest city in Colbert County, with a population of 17,772.
Belgreen is the smallest city in Colbert County.
There are 8 cities in Colbert County, Alabama.