16 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Strawberry | 1 | 5,692 | 5,325 | 0.35% | 4,280 | 1 | CDP |
| Sonora | 2 | 4,861 | 5,001 | -0.57% | 1,537 | 3 | City |
| Phoenix Lake | 3 | 4,404 | 4,820 | 0.2% | 398 | 11 | CDP |
| Jamestown | 4 | 3,819 | 3,379 | 0.66% | 1,272 | 3 | CDP |
| Mono Vista | 5 | 3,743 | 3,646 | 1.11% | 1,324 | 3 | CDP |
| East Sonora | 6 | 2,763 | 2,672 | 1.21% | 1,130 | 2 | CDP |
| Columbia | 7 | 2,475 | 2,086 | 0.49% | 415 | 6 | CDP |
| Twain Harte | 8 | 2,136 | 2,502 | -0.28% | 577 | 4 | CDP |
| Soulsbyville | 9 | 1,911 | 2,063 | -1.09% | 635 | 3 | CDP |
| Tuolumne City | 10 | 1,176 | 1,414 | -3.37% | 499 | 2 | CDP |
| Mi-Wuk Village | 11 | 1,027 | 933 | 0.49% | 362 | 3 | CDP |
| Tuttletown | 12 | 1,015 | 770 | 2.32% | 138 | 7 | CDP |
| Groveland | 13 | 588 | 249 | -0.17% | 61 | 10 | CDP |
| Strawberry | 14 | 80 | 5 | 6.67% | 154 | 1 | CDP |
| Chinese Camp | 15 | 36 | 146 | -14.29% | 40 | 1 | CDP |
| Long Barn | 16 | 14 | 88 | -39.13% | 5 | 3 | CDP |
Strawberry is the largest city in Tuolumne County, with a population of 5,692.
Long Barn is the smallest city in Tuolumne County, with a population of 14.
There are 16 cities in Tuolumne County, California.