16 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Lakeland | 1 | 127,767 | 113,881 | 2.22% | 1,920 | 67 | City |
| Winter Haven | 2 | 62,318 | 50,603 | 3.91% | 1,868 | 33 | City |
| Haines City | 3 | 45,604 | 27,949 | 8.39% | 1,911 | 24 | City |
| Bartow | 4 | 22,711 | 19,574 | 2.84% | 486 | 47 | City |
| Auburndale | 5 | 21,888 | 15,742 | 5.95% | 1,614 | 14 | City |
| Davenport | 6 | 18,522 | 9,730 | 10.49% | 4,211 | 4 | City |
| Lake Wales | 7 | 18,175 | 16,290 | 2.12% | 895 | 20 | City |
| Lake Alfred | 8 | 9,155 | 6,539 | 6.06% | 436 | 21 | City |
| Dundee | 9 | 6,525 | 5,273 | 3.98% | 584 | 11 | Town |
| Eagle Lake | 10 | 6,206 | 3,055 | 11.3% | 2,390 | 3 | City |
| Fort Meade | 11 | 5,401 | 5,101 | 1.12% | 660 | 8 | City |
| Mulberry | 12 | 4,619 | 3,964 | 2.92% | 679 | 7 | City |
| Frostproof | 13 | 3,530 | 3,214 | 1.82% | 303 | 12 | City |
| Polk City | 14 | 3,152 | 2,772 | 2.47% | 700 | 5 | Town |
| Lake Hamilton | 15 | 2,269 | 1,552 | 6.73% | 480 | 5 | Town |
| Hillcrest Heights | 16 | 290 | 250 | 2.84% | 1,813 | 0 | Town |
Lakeland is the largest city in Polk County, with a population of 127,767.
Hillcrest Heights is the smallest city in Polk County, with a population of 290.
There are 16 cities in Polk County, Florida.