21 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Deltona | 1 | 101,869 | 94,044 | 1.56% | 2,729 | 37.32 | City |
| Daytona Beach | 2 | 89,290 | 72,277 | 3.96% | 1,325 | 67.38 | City |
| Port Orange | 3 | 68,124 | 62,729 | 1.61% | 2,539 | 26.83 | City |
| DeLand | 4 | 46,206 | 38,213 | 3.58% | 2,375 | 19.45 | City |
| Ormond Beach | 5 | 45,050 | 43,117 | 0.87% | 1,289 | 34.96 | City |
| New Smyrna Beach | 6 | 34,149 | 30,413 | 2.24% | 902 | 37.84 | City |
| Edgewater | 7 | 23,964 | 23,143 | 0.69% | 966 | 24.80 | City |
| DeBary | 8 | 23,809 | 22,308 | 1.28% | 1,256 | 18.95 | City |
| Orange City | 9 | 16,412 | 12,701 | 4.74% | 2,100 | 7.82 | City |
| South Daytona | 10 | 14,317 | 12,976 | 1.91% | 3,868 | 3.70 | City |
| Holly Hill | 11 | 13,142 | 12,974 | 0.26% | 3,317 | 3.96 | City |
| Ormond-by-the-Sea | 12 | 6,484 | 6,999 | -0.93% | 3,216 | 2.02 | CDP |
| Daytona Beach Shores | 13 | 5,241 | 5,123 | 0.46% | 6,360 | 0.82 | City |
| Samsula-Spruce Creek | 14 | 5,160 | 5,016 | 0.16% | 307 | 16.81 | CDP |
| Ponce Inlet | 15 | 3,503 | 3,370 | 0.78% | 815 | 4.30 | Town |
| Lake Helen | 16 | 3,043 | 2,845 | 1.33% | 668 | 4.55 | City |
| De Leon Springs | 17 | 2,778 | 2,982 | 0.4% | 1,030 | 2.70 | CDP |
| Oak Hill | 18 | 2,201 | 2,018 | 1.71% | 314 | 7.02 | City |
| Pierson | 19 | 1,569 | 1,541 | 0.38% | 154 | 10.20 | Town |
| Seville | 20 | 1,484 | 917 | 4.07% | 209 | 7.10 | CDP |
| Glencoe | 21 | 1,127 | 1,439 | -7.92% | 332 | 3.39 | CDP |
Deltona is the largest city in Volusia County, with a population of 101,869.
Glencoe is the smallest city in Volusia County.
There are 21 cities in Volusia County, Florida.