12 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Moultrie | 1 | 14,791 | 14,621 | 0.24% | 887 | 17 | City |
| Moultrie | 2 | 14,791 | 14,614 | 0.24% | 887 | 17 | City |
| Norman Park | 3 | 1,009 | 966 | 0.8% | 328 | 3 | City |
| Norman Park | 4 | 1,009 | 967 | 0.8% | 328 | 3 | City |
| Doerun | 5 | 726 | 739 | -0.41% | 565 | 1 | City |
| Doerun | 6 | 726 | 738 | -0.41% | 565 | 1 | City |
| Berlin | 7 | 531 | 511 | 0.57% | 635 | 1 | City |
| Berlin | 8 | 531 | 513 | 0.57% | 635 | 1 | City |
| Funston | 9 | 425 | 405 | 0.95% | 372 | 1 | City |
| Funston | 10 | 425 | 404 | 0.95% | 372 | 1 | City |
| Ellenton | 11 | 221 | 211 | 0.91% | 279 | 1 | Town |
| Ellenton | 12 | 221 | 211 | 0.91% | 279 | 1 | Town |
Moultrie is the largest city in Colquitt County, with a population of 14,791.
Ellenton is the smallest city in Colquitt County, with a population of 221.
There are 12 cities in Colquitt County, Georgia.