13 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Louisville | 1 | 2,184 | 2,384 | -1.75% | 605 | 4 | City |
| Louisville | 2 | 2,184 | 2,377 | -1.75% | 605 | 4 | City |
| Wrens | 3 | 2,070 | 2,219 | -1.38% | 660 | 3 | City |
| Wrens | 4 | 2,070 | 2,214 | -1.38% | 660 | 3 | City |
| Wadley | 5 | 1,559 | 1,644 | -1.08% | 342 | 5 | City |
| Wadley | 6 | 1,559 | 1,641 | -1.08% | 342 | 5 | City |
| Stapleton | 7 | 396 | 400 | -0.25% | 227 | 2 | City |
| Stapleton | 8 | 396 | 399 | -0.25% | 227 | 2 | City |
| Avera | 9 | 212 | 223 | -0.93% | 326 | 1 | City |
| Avera | 10 | 212 | 222 | -0.93% | 326 | 1 | City |
| Matthews | 11 | 194 | 103 | 1.57% | 55 | 4 | CDP |
| Bartow | 12 | 176 | 185 | -1.12% | 156 | 1 | Town |
| Bartow | 13 | 176 | 185 | -1.12% | 156 | 1 | Town |
Louisville is the largest city in Jefferson County, with a population of 2,184.
Bartow is the smallest city in Jefferson County, with a population of 176.
There are 13 cities in Jefferson County, Georgia.