23 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Carrollton | 1 | 2,746 | 2,888 | -0.87% | 62 | 44 | Township |
| White Hall | 2 | 2,387 | 2,505 | -0.79% | 57 | 42 | Township |
| Carrollton | 3 | 2,341 | 2,473 | -1.1% | 1,231 | 2 | City |
| White Hall | 4 | 2,152 | 2,274 | -1.1% | 835 | 3 | City |
| Roodhouse | 5 | 1,774 | 1,865 | -0.78% | 45 | 40 | Township |
| Rockbridge | 6 | 1,481 | 1,557 | -0.8% | 31 | 48 | Township |
| Roodhouse | 7 | 1,480 | 1,569 | -1.2% | 1,313 | 1 | City |
| Greenfield | 8 | 1,015 | 992 | 0.4% | 59 | 17 | Township |
| Greenfield | 9 | 993 | 1,053 | -1.19% | 579 | 2 | City |
| Kane | 10 | 733 | 772 | -0.81% | 15 | 49 | Township |
| Bluffdale | 11 | 432 | 452 | -0.69% | 10 | 45 | Township |
| Patterson | 12 | 390 | 404 | -0.51% | 8 | 47 | Township |
| Linder | 13 | 295 | 318 | -1.67% | 9 | 35 | Township |
| Rubicon | 14 | 280 | 296 | -1.06% | 8 | 36 | Township |
| Kane | 15 | 276 | 296 | -1.43% | 507 | 1 | Village |
| Athensville | 16 | 270 | 283 | -0.74% | 8 | 36 | Township |
| Wrights | 17 | 208 | 225 | -1.89% | 6 | 36 | Township |
| Woodville | 18 | 197 | 208 | -1.01% | 4 | 48 | Township |
| Rockbridge | 19 | 167 | 176 | -1.18% | 227 | 1 | Village |
| Walkerville | 20 | 156 | 163 | -0.64% | 4 | 39 | Township |
| Eldred | 21 | 143 | 150 | -0.69% | 1,067 | 0 | Village |
| Hillview | 22 | 88 | 92 | -1.12% | 108 | 1 | Village |
| Otterville | 23 | 81 | 86 | -1.22% | 81 | 1 | Town |
Carrollton is the largest city in Greene County, with a population of 2,746.
Otterville is the smallest city in Greene County, with a population of 81.
There are 23 cities in Greene County, Illinois.