13 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Jasper | 1 | 17,091 | 16,718 | 0.36% | 1,285 | 13 | City |
| Huntingburg | 2 | 6,567 | 6,401 | 0.43% | 1,299 | 5 | City |
| Ferdinand | 3 | 3,572 | 3,611 | -0.53% | 96 | 37 | Township |
| Ferdinand | 4 | 2,204 | 2,155 | 0.36% | 960 | 2 | Town |
| Jackson | 5 | 2,114 | 2,194 | -0.89% | 61 | 35 | Township |
| Cass | 6 | 1,999 | 2,082 | -0.94% | 52 | 39 | Township |
| Jefferson | 7 | 1,536 | 1,599 | -0.9% | 43 | 36 | Township |
| Harbison | 8 | 1,516 | 1,576 | -0.92% | 40 | 38 | Township |
| Hall | 9 | 1,236 | 1,286 | -0.88% | 37 | 33 | Township |
| Columbia | 10 | 964 | 1,008 | -0.92% | 27 | 36 | Township |
| Boone | 11 | 919 | 943 | -0.54% | 28 | 33 | Township |
| Holland | 12 | 596 | 620 | -0.67% | 1,598 | 0 | Town |
| Birdseye | 13 | 406 | 417 | -0.49% | 638 | 1 | Town |
Jasper is the largest city in Dubois County, with a population of 17,091.
Birdseye is the smallest city in Dubois County, with a population of 406.
There are 13 cities in Dubois County, Indiana.