28 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Henry | 1 | 22,005 | 21,988 | 0.16% | 604 | 36 | Township |
| New Castle | 2 | 17,283 | 17,398 | -0.13% | 2,360 | 7 | City |
| Prairie | 3 | 6,016 | 5,989 | 0.2% | 151 | 40 | Township |
| Fall Creek | 4 | 4,713 | 4,652 | 0.38% | 155 | 30 | Township |
| Wayne | 5 | 4,155 | 4,118 | 0.27% | 128 | 32 | Township |
| Middletown | 6 | 2,272 | 2,252 | 0.18% | 1,960 | 1 | Town |
| Spiceland | 7 | 2,193 | 2,172 | 0.32% | 99 | 22 | Township |
| Knightstown | 8 | 2,157 | 2,139 | 0.19% | 2,068 | 1 | Town |
| Greensboro | 9 | 1,659 | 1,643 | 0.36% | 65 | 26 | Township |
| Harrison | 10 | 1,413 | 1,394 | 0.43% | 40 | 36 | Township |
| Jefferson | 11 | 1,390 | 1,379 | 0.29% | 49 | 28 | Township |
| Blue River | 12 | 1,154 | 1,147 | 0.17% | 53 | 22 | Township |
| Dudley | 13 | 1,112 | 1,100 | 0.36% | 36 | 31 | Township |
| Spiceland | 14 | 955 | 957 | 0% | 655 | 1 | Town |
| Shirley | 15 | 822 | 821 | 0% | 2,277 | 0 | Town |
| Stoney Creek | 16 | 812 | 805 | 0.25% | 42 | 19 | Township |
| Kennard | 17 | 447 | 454 | -0.22% | 1,057 | 0 | Town |
| Mount Summit | 18 | 350 | 349 | 0% | 1,699 | 0 | Town |
| Lewisville | 19 | 340 | 339 | 0% | 1,323 | 0 | Town |
| Mooreland | 20 | 338 | 337 | 0% | 2,364 | 0 | Town |
| Sulphur Springs | 21 | 335 | 334 | 0% | 477 | 1 | Town |
| East Germantown | 22 | 262 | 262 | 0% | 2,239 | 0 | Town |
| Straughn | 23 | 261 | 258 | 0.38% | 1,851 | 0 | Town |
| Dunreith | 24 | 171 | 168 | 0.59% | 1,379 | 0 | Town |
| Cadiz | 25 | 162 | 162 | 0% | 1,125 | 0 | Town |
| Springport | 26 | 139 | 136 | 0.72% | 1,158 | 0 | Town |
| Greensboro | 27 | 126 | 123 | 0.8% | 1,145 | 0 | Town |
| Mount Auburn | 28 | 124 | 126 | 0% | 653 | 0 | Town |
Henry is the largest city in Henry County, with a population of 22,005.
Mount Auburn is the smallest city in Henry County, with a population of 124.
There are 28 cities in Henry County, Indiana.