9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Stuart | 1 | 1,909 | 1,796 | 1.17% | 678 | 3 | City |
| Guthrie Center | 2 | 1,578 | 1,583 | -0.06% | 663 | 2 | City |
| Panora | 3 | 1,098 | 1,088 | 0.18% | 606 | 2 | City |
| Bayard | 4 | 401 | 405 | -0.25% | 786 | 1 | City |
| Casey | 5 | 387 | 385 | 0% | 524 | 1 | City |
| Menlo | 6 | 347 | 344 | 0% | 700 | 0 | City |
| Yale | 7 | 264 | 267 | -0.38% | 913 | 0 | City |
| Bagley | 8 | 229 | 232 | -0.43% | 732 | 0 | City |
| Jamaica | 9 | 196 | 194 | 0% | 527 | 0 | City |
Stuart is the largest city in Guthrie County, with a population of 1,909.
Jamaica is the smallest city in Guthrie County, with a population of 196.
There are 9 cities in Guthrie County, Iowa.