17 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Crowley | 1 | 11,100 | 11,698 | -1.03% | 1,897 | 6 | City |
| Crowley | 2 | 11,100 | 11,678 | -1.03% | 1,897 | 6 | City |
| Rayne | 3 | 6,918 | 7,254 | -0.93% | 1,824 | 4 | City |
| Rayne | 4 | 6,918 | 7,243 | -0.93% | 1,824 | 4 | City |
| Church Point | 5 | 3,996 | 4,182 | -0.89% | 1,381 | 3 | Town |
| Church Point | 6 | 3,996 | 4,175 | -0.89% | 1,381 | 3 | Town |
| Iota | 7 | 1,244 | 1,308 | -1.03% | 980 | 1 | Town |
| Iota | 8 | 1,244 | 1,306 | -1.03% | 980 | 1 | Town |
| Egan | 9 | 884 | 1,002 | 1.96% | 182 | 5 | CDP |
| Estherwood | 10 | 674 | 697 | -0.74% | 362 | 2 | Village |
| Estherwood | 11 | 674 | 698 | -0.74% | 362 | 2 | Village |
| Morse | 12 | 599 | 594 | 0.17% | 428 | 1 | Village |
| Morse | 13 | 599 | 593 | 0.17% | 428 | 1 | Village |
| Branch | 14 | 490 | 355 | 1.24% | 67 | 7 | CDP |
| Mermentau | 15 | 490 | 520 | -1.21% | 230 | 2 | Village |
| Mermentau | 16 | 490 | 519 | -1.21% | 230 | 2 | Village |
| Midland | 17 | 270 | 173 | 7.14% | 1,343 | 0 | CDP |
Crowley is the largest city in Acadia Parish, with a population of 11,100.
Midland is the smallest city in Acadia Parish, with a population of 270.
There are 17 cities in Acadia Parish, Louisiana.