21 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Omaha | 1 | 488,385 | 491,875 | -0.18% | 3,416 | 143 | City |
| Omaha | 2 | 488,385 | 492,783 | -0.18% | 3,416 | 143 | City |
| Ralston | 3 | 6,432 | 6,488 | -0.17% | 3,936 | 2 | City |
| Ralston | 4 | 6,432 | 6,485 | -0.17% | 3,936 | 2 | City |
| Valley | 5 | 3,408 | 3,036 | 2.16% | 703 | 5 | City |
| Valley | 6 | 3,408 | 3,047 | 2.16% | 703 | 5 | City |
| Bennington | 7 | 1,972 | 2,022 | -0.5% | 2,198 | 1 | City |
| Bennington | 8 | 1,972 | 2,022 | -0.5% | 2,198 | 1 | City |
| Omaha | 9 | 1,033 | 1,022 | 0.98% | 23 | 45 | Township |
| Omaha | 10 | 1,033 | 1,029 | 0.98% | 23 | 45 | Township |
| Waterloo | 11 | 919 | 939 | -0.43% | 1,143 | 1 | Village |
| Waterloo | 12 | 919 | 939 | -0.43% | 1,143 | 1 | Village |
| Boys Town | 13 | 706 | 420 | 8.78% | 778 | 1 | Village |
| Boys Town | 14 | 706 | 420 | 8.78% | 778 | 1 | Village |
| Elkhorn | 15 | 333 | 342 | 0% | 11 | 31 | Township |
| Elkhorn | 16 | 333 | 340 | 0% | 11 | 31 | Township |
| Elkhorn | 17 | 241 | 244 | -0.41% | 7 | 36 | Township |
| Elkhorn | 18 | 241 | 244 | -0.41% | 7 | 36 | Township |
| Valley | 19 | 142 | 146 | -0.7% | 4 | 36 | Township |
| Valley | 20 | 142 | 146 | -0.7% | 4 | 36 | Township |
| Venice | 21 | 32 | 45 | 6.67% | 68 | 0 | CDP |
Omaha is the largest city in Douglas County, with a population of 488,385.
Venice is the smallest city in Douglas County, with a population of 32.
There are 21 cities in Douglas County, Nebraska.