26 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Hebron | 1 | 1,383 | 1,456 | -1% | 938 | 1 | City |
| Hebron | 2 | 1,383 | 1,453 | -1% | 938 | 1 | City |
| Deshler | 3 | 740 | 742 | 0% | 1,457 | 1 | City |
| Deshler | 4 | 740 | 740 | 0% | 1,457 | 1 | City |
| Chester | 5 | 384 | 381 | -1.29% | 13 | 30 | Township |
| Chester | 6 | 384 | 383 | -1.29% | 13 | 30 | Township |
| Davenport | 7 | 304 | 319 | -0.98% | 466 | 1 | Village |
| Davenport | 8 | 304 | 318 | -0.98% | 466 | 1 | Village |
| Bruning | 9 | 278 | 292 | -1.07% | 858 | 0 | Village |
| Bruning | 10 | 278 | 291 | -1.07% | 858 | 0 | Village |
| Chester | 11 | 209 | 222 | -1.42% | 357 | 1 | Village |
| Chester | 12 | 209 | 221 | -1.42% | 357 | 1 | Village |
| Alexandria | 13 | 139 | 146 | -1.42% | 344 | 0 | Village |
| Alexandria | 14 | 139 | 146 | -1.42% | 344 | 0 | Village |
| Carleton | 15 | 92 | 96 | -1.08% | 116 | 1 | Village |
| Carleton | 16 | 92 | 96 | -1.08% | 116 | 1 | Village |
| Byron | 17 | 86 | 90 | -1.15% | 551 | 0 | Village |
| Byron | 18 | 86 | 90 | -1.15% | 551 | 0 | Village |
| Hubbell | 19 | 66 | 69 | -1.49% | 212 | 0 | Village |
| Hubbell | 20 | 66 | 69 | -1.49% | 212 | 0 | Village |
| Reynolds | 21 | 59 | 61 | -1.67% | 235 | 0 | Village |
| Reynolds | 22 | 59 | 61 | -1.67% | 235 | 0 | Village |
| Belvidere | 23 | 54 | 57 | -1.82% | 116 | 0 | Village |
| Belvidere | 24 | 54 | 57 | -1.82% | 116 | 0 | Village |
| Gilead | 25 | 29 | 31 | -3.33% | 420 | 0 | Village |
| Gilead | 26 | 29 | 31 | -3.33% | 420 | 0 | Village |
Hebron is the largest city in Thayer County, with a population of 1,383.
Gilead is the smallest city in Thayer County, with a population of 29.
There are 26 cities in Thayer County, Nebraska.