34 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Oneonta | 1 | 16,335 | 13,054 | 4.18% | 3,746 | 4 | City |
| Oneonta | 2 | 4,934 | 5,057 | -0.32% | 150 | 33 | Town |
| Unadilla | 3 | 4,073 | 4,101 | 0.12% | 88 | 46 | Town |
| Otsego | 4 | 3,649 | 3,637 | 0.58% | 68 | 54 | Town |
| Milford | 5 | 2,755 | 2,817 | 0% | 60 | 46 | Town |
| Otego | 6 | 2,684 | 2,751 | -0.33% | 59 | 46 | Town |
| Laurens | 7 | 2,301 | 2,303 | 0.22% | 55 | 42 | Town |
| Worcester | 8 | 2,056 | 2,103 | -0.24% | 44 | 47 | Town |
| Richfield | 9 | 2,051 | 2,059 | 0.2% | 66 | 31 | Town |
| Hartwick | 10 | 1,943 | 1,947 | 0.21% | 48 | 40 | Town |
| Edmeston | 11 | 1,891 | 1,901 | 0.11% | 43 | 44 | Town |
| Middlefield | 12 | 1,862 | 1,876 | 0.16% | 29 | 63 | Town |
| Cooperstown | 13 | 1,842 | 1,852 | -0.11% | 1,127 | 2 | Village |
| Maryland | 14 | 1,748 | 1,751 | 0.17% | 33 | 52 | Town |
| Morris | 15 | 1,719 | 1,727 | 0.12% | 44 | 39 | Town |
| Butternuts | 16 | 1,652 | 1,658 | 0.12% | 31 | 54 | Town |
| Springfield | 17 | 1,326 | 1,337 | 0.08% | 31 | 43 | Town |
| Pittsfield | 18 | 1,299 | 1,310 | 0% | 34 | 38 | Town |
| Cherry Valley | 19 | 1,229 | 1,232 | 0.33% | 30 | 40 | Town |
| Richfield Springs | 20 | 1,088 | 1,098 | -0.18% | 1,080 | 1 | Village |
| Unadilla | 21 | 1,084 | 1,092 | -0.18% | 1,045 | 1 | Village |
| New Lisbon | 22 | 1,073 | 1,077 | 0.19% | 24 | 44 | Town |
| Burlington | 23 | 1,035 | 1,040 | 0.1% | 23 | 45 | Town |
| Plainfield | 24 | 920 | 923 | 0.22% | 31 | 30 | Town |
| Otego | 25 | 905 | 935 | -0.66% | 781 | 1 | Village |
| Exeter | 26 | 834 | 842 | 0% | 26 | 32 | Town |
| Westford | 27 | 810 | 808 | 0.37% | 24 | 34 | Town |
| Roseboom | 28 | 664 | 685 | -0.45% | 20 | 33 | Town |
| Morris | 29 | 516 | 519 | -0.19% | 692 | 1 | Village |
| Cherry Valley | 30 | 477 | 480 | -0.21% | 935 | 1 | Village |
| Milford | 31 | 393 | 396 | -0.25% | 927 | 0 | Village |
| Decatur | 32 | 368 | 370 | 0% | 18 | 21 | Town |
| Gilbertsville | 33 | 327 | 330 | -0.3% | 326 | 1 | Village |
| Laurens | 34 | 219 | 218 | 0% | 1,711 | 0 | Village |
Oneonta is the largest city in Otsego County, with a population of 16,335.
Laurens is the smallest city in Otsego County, with a population of 219.
There are 34 cities in Otsego County, New York.