31 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Arcadia | 1 | 13,495 | 13,720 | -0.18% | 259 | 52 | Town |
| Ontario | 2 | 10,542 | 10,454 | 0.25% | 325 | 32 | Town |
| Walworth | 3 | 9,458 | 9,247 | 0.91% | 279 | 34 | Town |
| Macedon | 4 | 9,318 | 9,265 | 0.58% | 241 | 39 | Town |
| Newark | 5 | 8,895 | 9,078 | -0.41% | 1,641 | 5 | Village |
| Sodus | 6 | 8,100 | 8,031 | 0.04% | 120 | 67 | Town |
| Palmyra | 7 | 7,305 | 7,393 | -0.16% | 218 | 33 | Town |
| Williamson | 8 | 6,786 | 6,850 | -0.06% | 196 | 35 | Town |
| Lyons | 9 | 5,526 | 5,658 | -0.18% | 147 | 37 | Town |
| Lyons | 10 | 4,492 | 3,313 | 7.13% | 974 | 5 | CDP |
| Marion | 11 | 4,488 | 4,562 | -0.24% | 154 | 29 | Town |
| Galen | 12 | 4,344 | 4,410 | -0.09% | 73 | 59 | Town |
| Wolcott | 13 | 3,947 | 4,004 | -0.15% | 101 | 39 | Town |
| Palmyra | 14 | 3,249 | 3,318 | -0.43% | 2,412 | 1 | Village |
| Williamson | 15 | 2,576 | 2,642 | 0.19% | 671 | 4 | CDP |
| Rose | 16 | 2,253 | 2,296 | -0.13% | 66 | 34 | Town |
| Clyde | 17 | 2,116 | 2,166 | -0.47% | 963 | 2 | Village |
| Ontario | 18 | 2,113 | 2,038 | -0.14% | 524 | 4 | CDP |
| Macedon | 19 | 2,085 | 1,794 | 7.14% | 958 | 2 | CDP |
| Huron | 20 | 1,860 | 1,865 | 0.05% | 47 | 39 | Town |
| Butler | 21 | 1,804 | 1,823 | -0.11% | 49 | 37 | Town |
| Sodus | 22 | 1,689 | 1,718 | -0.35% | 1,795 | 1 | Village |
| Savannah | 23 | 1,617 | 1,635 | -0.43% | 45 | 36 | Town |
| Wolcott | 24 | 1,568 | 1,602 | -0.44% | 805 | 2 | Village |
| Marion | 25 | 1,146 | 1,251 | -2.05% | 375 | 3 | CDP |
| Walworth | 26 | 1,081 | 722 | 7.14% | 737 | 1 | CDP |
| Sodus Point | 27 | 849 | 823 | 0.59% | 579 | 1 | Village |
| Pultneyville | 28 | 673 | 655 | -0.3% | 287 | 2 | CDP |
| North Rose | 29 | 576 | 664 | -0.69% | 341 | 2 | CDP |
| Red Creek | 30 | 485 | 493 | -0.41% | 534 | 1 | Village |
| Savannah | 31 | 362 | 550 | -3.47% | 307 | 1 | CDP |
Arcadia is the largest city in Wayne County, with a population of 13,495.
Savannah is the smallest city in Wayne County, with a population of 362.
There are 31 cities in Wayne County, New York.