25 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Athens township | 1 | 34,764 | 30,142 | 5.88% | 950 | 36.59 | Township |
| Athens | 2 | 25,239 | 23,824 | 1.13% | 2,538 | 9.95 | City |
| York township | 3 | 6,686 | 6,838 | -0.42% | 181 | 36.88 | Township |
| Nelsonville | 4 | 4,464 | 4,598 | -0.6% | 954 | 4.68 | City |
| Trimble township | 5 | 3,966 | 4,060 | -0.38% | 107 | 37.07 | Township |
| Lee township | 6 | 2,814 | 2,843 | -0.04% | 113 | 24.92 | Township |
| Alexander township | 7 | 2,754 | 2,799 | -0.25% | 74 | 37.06 | Township |
| The Plains | 8 | 2,635 | 2,813 | -1.13% | 1,156 | 2.28 | CDP |
| Glouster | 9 | 1,608 | 1,660 | -0.62% | 1,228 | 1.31 | Village |
| Canaan township | 10 | 1,598 | 1,631 | -0.31% | 42 | 38.06 | Township |
| Carthage township | 11 | 1,465 | 1,490 | -0.2% | 38 | 38.55 | Township |
| Ames township | 12 | 1,214 | 1,234 | -0.25% | 32 | 37.73 | Township |
| Chauncey | 13 | 933 | 960 | -0.53% | 1,696 | 0.55 | Village |
| Albany | 14 | 922 | 926 | -0.11% | 692 | 1.33 | Village |
| Bern township | 15 | 555 | 568 | -0.36% | 18 | 31.28 | Township |
| Buchtel | 16 | 509 | 519 | -0.39% | 1,083 | 0.47 | Village |
| Coolville | 17 | 440 | 452 | -0.45% | 449 | 0.98 | Village |
| Jacksonville | 18 | 390 | 404 | -0.76% | 1,625 | 0.24 | Village |
| Athens township | 19 | 352 | 372 | -1.4% | 14 | 25.10 | Township |
| Stewart | 20 | 338 | 326 | 1.81% | 1,142 | 0.30 | CDP |
| Trimble | 21 | 320 | 325 | -0.31% | 471 | 0.68 | Village |
| Millfield | 22 | 237 | 293 | -2.87% | 418 | 0.57 | CDP |
| Amesville | 23 | 168 | 173 | -0.59% | 740 | 0.23 | Village |
| New Marshfield | 24 | 148 | 236 | -7.5% | 378 | 0.39 | CDP |
| Hockingport | 25 | 15 | 144 | -46.43% | 35 | 0.43 | CDP |
Athens township is the largest city in Athens County, with a population of 34,764.
Hockingport is the smallest city in Athens County.
There are 25 cities in Athens County, Ohio.