9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Millersburg | 1 | 3,210 | 3,149 | 0.38% | 1,266 | 2.54 | Village |
| Walnut Creek | 2 | 1,408 | 1,437 | 2.55% | 633 | 2.22 | CDP |
| Berlin | 3 | 1,284 | 1,152 | 2.07% | 336 | 3.82 | CDP |
| Killbuck | 4 | 803 | 805 | 0% | 2,224 | 0.36 | Village |
| Holmesville | 5 | 373 | 373 | 0% | 1,673 | 0.22 | Village |
| Glenmont | 6 | 239 | 236 | 0.42% | 869 | 0.28 | Village |
| Winesburg | 7 | 211 | 287 | -4.09% | 337 | 0.63 | CDP |
| Mount Hope | 8 | 185 | 120 | 6.94% | 217 | 0.85 | CDP |
| Nashville | 9 | 183 | 182 | 0% | 2,652 | 0.07 | Village |
Millersburg is the largest city in Holmes County, with a population of 3,210.
Nashville is the smallest city in Holmes County.
There are 9 cities in Holmes County, Ohio.