21 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Berlin | 1 | 10,970 | 9,377 | 2.08% | 491 | 22 | Township |
| Hardy | 2 | 6,189 | 5,933 | 1.11% | 188 | 33 | Township |
| Salt Creek | 3 | 4,739 | 4,668 | 0.36% | 158 | 30 | Township |
| Berlin | 4 | 4,645 | 4,575 | 0.39% | 179 | 26 | Township |
| Paint | 5 | 4,614 | 4,542 | 0.44% | 160 | 29 | Township |
| Clark | 6 | 4,393 | 4,329 | 0.41% | 127 | 34 | Township |
| Walnut Creek | 7 | 4,057 | 4,002 | 0.35% | 146 | 28 | Township |
| Berlin | 8 | 3,401 | 3,437 | 0.09% | 112 | 30 | Township |
| Millersburg | 9 | 3,313 | 3,166 | 0.76% | 1,306 | 3 | Village |
| Mechanic | 10 | 3,274 | 3,217 | 0.46% | 97 | 34 | Township |
| Prairie | 11 | 3,105 | 3,051 | 0.42% | 102 | 31 | Township |
| Berlin | 12 | 1,951 | 1,965 | -0.05% | 81 | 24 | Township |
| Killbuck | 13 | 1,927 | 1,889 | 0.47% | 64 | 30 | Township |
| Berlin | 14 | 1,785 | 1,787 | -0.28% | 97 | 18 | Township |
| Washington | 15 | 1,676 | 1,638 | 0.54% | 57 | 30 | Township |
| Richland | 16 | 1,217 | 1,195 | 0.33% | 33 | 37 | Township |
| Knox | 17 | 1,186 | 1,160 | 0.59% | 43 | 28 | Township |
| Killbuck | 18 | 814 | 808 | 0.12% | 2,255 | 0 | Village |
| Holmesville | 19 | 376 | 373 | 0.27% | 1,686 | 0 | Village |
| Glenmont | 20 | 243 | 239 | 0.41% | 884 | 0 | Village |
| Nashville | 21 | 182 | 182 | 0% | 2,638 | 0 | Village |
Berlin is the largest city in Holmes County, with a population of 10,970.
Nashville is the smallest city in Holmes County, with a population of 182.
There are 21 cities in Holmes County, Ohio.