62 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Steubenville | 1 | 17,999 | 18,169 | -0.17% | 1,708 | 11 | City |
| Steubenville | 2 | 17,999 | 18,149 | -0.17% | 1,708 | 11 | City |
| Island Creek | 3 | 9,970 | 10,255 | -0.34% | 239 | 42 | Township |
| Island Creek | 4 | 9,970 | 10,244 | -0.34% | 239 | 42 | Township |
| Cross Creek | 5 | 7,609 | 7,844 | -0.35% | 243 | 31 | Township |
| Cross Creek | 6 | 7,609 | 7,837 | -0.35% | 243 | 31 | Township |
| Toronto | 7 | 5,158 | 5,305 | -0.56% | 2,473 | 2 | City |
| Toronto | 8 | 5,158 | 5,300 | -0.56% | 2,473 | 2 | City |
| Knox | 9 | 4,212 | 4,312 | -0.26% | 145 | 29 | Township |
| Knox | 10 | 4,212 | 4,310 | -0.26% | 145 | 29 | Township |
| Steubenville | 11 | 3,973 | 4,107 | -0.4% | 530 | 7 | Township |
| Steubenville | 12 | 3,973 | 4,102 | -0.4% | 530 | 7 | Township |
| Wintersville | 13 | 3,648 | 3,771 | -0.65% | 1,167 | 3 | Village |
| Wintersville | 14 | 3,648 | 3,768 | -0.65% | 1,167 | 3 | Village |
| Dillonvale | 15 | 3,277 | 3,411 | -0.4% | 3,674 | 1 | CDP |
| Mingo Junction | 16 | 3,227 | 3,346 | -0.71% | 1,200 | 3 | Village |
| Mingo Junction | 17 | 3,227 | 3,342 | -0.71% | 1,200 | 3 | Village |
| Smithfield | 18 | 2,919 | 3,006 | -0.31% | 78 | 38 | Township |
| Smithfield | 19 | 2,919 | 3,002 | -0.31% | 78 | 38 | Township |
| Wells | 20 | 2,381 | 2,448 | -0.29% | 89 | 27 | Township |
| Wells | 21 | 2,381 | 2,446 | -0.29% | 89 | 27 | Township |
| Mount Pleasant | 22 | 2,152 | 2,228 | -0.42% | 113 | 19 | Township |
| Mount Pleasant | 23 | 2,152 | 2,225 | -0.42% | 113 | 19 | Township |
| Tiltonsville | 24 | 1,211 | 1,258 | -0.74% | 2,311 | 1 | Village |
| Tiltonsville | 25 | 1,211 | 1,255 | -0.74% | 2,311 | 1 | Village |
| Saline | 26 | 1,126 | 1,160 | -0.44% | 53 | 21 | Township |
| Saline | 27 | 1,126 | 1,159 | -0.44% | 53 | 21 | Township |
| Richmond | 28 | 997 | 1,012 | -0.3% | 39 | 25 | Township |
| Richmond | 29 | 997 | 1,011 | -0.3% | 39 | 25 | Township |
| Brilliant | 30 | 964 | 1,302 | -3.5% | 552 | 2 | CDP |
| Richmond | 31 | 945 | 971 | -0.53% | 37 | 25 | Township |
| Richmond | 32 | 945 | 970 | -0.53% | 37 | 25 | Township |
| Yorkville | 33 | 929 | 968 | -0.85% | 1,569 | 1 | Village |
| Yorkville | 34 | 929 | 966 | -0.85% | 1,569 | 1 | Village |
| Adena | 35 | 643 | 667 | -0.77% | 1,197 | 1 | Village |
| Adena | 36 | 643 | 667 | -0.77% | 1,197 | 1 | Village |
| Dillonvale | 37 | 592 | 615 | -0.84% | 1,534 | 0 | Village |
| Dillonvale | 38 | 592 | 615 | -0.84% | 1,534 | 0 | Village |
| Bergholz | 39 | 519 | 542 | -0.95% | 922 | 1 | Village |
| Bergholz | 40 | 519 | 542 | -0.95% | 922 | 1 | Village |
| Amsterdam | 41 | 422 | 437 | -0.71% | 1,319 | 0 | Village |
| Amsterdam | 42 | 422 | 437 | -0.71% | 1,319 | 0 | Village |
| Richmond | 43 | 398 | 412 | -0.75% | 720 | 1 | Village |
| Richmond | 44 | 398 | 412 | -0.75% | 720 | 1 | Village |
| Brush Creek | 45 | 382 | 392 | 0% | 16 | 24 | Township |
| Brush Creek | 46 | 382 | 392 | 0% | 16 | 24 | Township |
| Rayland | 47 | 379 | 393 | -0.79% | 801 | 0 | Village |
| Rayland | 48 | 379 | 393 | -0.79% | 801 | 0 | Village |
| Mount Pleasant | 49 | 377 | 392 | -0.79% | 1,478 | 0 | Village |
| Mount Pleasant | 50 | 377 | 392 | -0.79% | 1,478 | 0 | Village |
| Irondale | 51 | 315 | 327 | -0.94% | 217 | 1 | Village |
| Irondale | 52 | 315 | 327 | -0.94% | 217 | 1 | Village |
| Stratton | 53 | 260 | 265 | -0.38% | 484 | 1 | Village |
| Stratton | 54 | 260 | 265 | -0.38% | 484 | 1 | Village |
| Empire | 55 | 226 | 230 | -0.44% | 753 | 0 | Village |
| Empire | 56 | 226 | 230 | -0.44% | 753 | 0 | Village |
| New Alexandria | 57 | 223 | 232 | -0.89% | 608 | 0 | Village |
| New Alexandria | 58 | 223 | 232 | -0.89% | 608 | 0 | Village |
| East Springfield | 59 | 139 | 263 | 6.92% | 339 | 0 | CDP |
| Bloomingdale | 60 | 133 | 140 | -1.48% | 1,511 | 0 | Village |
| Bloomingdale | 61 | 133 | 140 | -1.48% | 1,511 | 0 | Village |
| Pottery Addition | 62 | 52 | 139 | -23.53% | 61 | 1 | CDP |
Steubenville is the largest city in Jefferson County, with a population of 17,999.
Pottery Addition is the smallest city in Jefferson County, with a population of 52.
There are 62 cities in Jefferson County, Ohio.