39 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Lemmon | 1 | 1,138 | 1,153 | -0.26% | 1,036 | 1 | City |
| Bison | 2 | 301 | 304 | -0.33% | 303 | 1 | Town |
| Lincoln | 3 | 145 | 145 | 0.69% | 2 | 81 | Township |
| De Witt | 4 | 43 | 44 | 0% | 1 | 56 | Township |
| Chance | 5 | 41 | 42 | 0% | 1 | 71 | Township |
| Horse Creek | 6 | 41 | 40 | 2.5% | 1 | 52 | Township |
| Meadow | 7 | 39 | 40 | 0% | 1 | 36 | Township |
| Marshfield | 8 | 37 | 36 | 2.78% | 1 | 72 | Township |
| Fredlund | 9 | 36 | 35 | 2.86% | 1 | 35 | Township |
| Lodgepole | 10 | 29 | 29 | 0% | 1 | 36 | Township |
| Burdick | 11 | 28 | 28 | 0% | 1 | 36 | Township |
| Wilson | 12 | 26 | 26 | 0% | 1 | 36 | Township |
| Lone Tree | 13 | 24 | 24 | 0% | 1 | 36 | Township |
| Rainbow | 14 | 24 | 24 | 0% | 1 | 35 | Township |
| Antelope | 15 | 23 | 23 | 0% | 1 | 36 | Township |
| Wells | 16 | 22 | 22 | 0% | 1 | 35 | Township |
| Foster | 17 | 21 | 21 | 0% | 0.00 | 64 | Township |
| Flat Creek | 18 | 20 | 20 | 0% | 1 | 36 | Township |
| Rockford | 19 | 20 | 20 | 0% | 1 | 35 | Township |
| Chaudoin | 20 | 19 | 19 | 0% | 1 | 36 | Township |
| Cash | 21 | 18 | 18 | 0% | 1 | 36 | Township |
| Grand River | 22 | 18 | 18 | 0% | 0.00 | 52 | Township |
| Scotch Cap | 23 | 18 | 18 | 0% | 1 | 35 | Township |
| Trail | 24 | 18 | 18 | 0% | 1 | 16 | Township |
| White Butte | 25 | 18 | 18 | 0% | 1 | 16 | Township |
| Barrett | 26 | 16 | 16 | 0% | 0.00 | 36 | Township |
| Bison | 27 | 16 | 16 | 0% | 0.00 | 35 | Township |
| Englewood | 28 | 16 | 16 | 0% | 0.00 | 65 | Township |
| Liberty | 29 | 16 | 16 | 0% | 0.00 | 36 | Township |
| Duell | 30 | 15 | 15 | 0% | 0.00 | 35 | Township |
| Maltby | 31 | 14 | 14 | 0% | 0.00 | 36 | Township |
| Moreau | 32 | 14 | 14 | 0% | 0.00 | 72 | Township |
| Vail | 33 | 13 | 13 | 0% | 0.00 | 36 | Township |
| Ada | 34 | 11 | 11 | 0% | 0.00 | 35 | Township |
| Beck-Highland | 35 | 11 | 11 | 0% | 0.00 | 72 | Township |
| Hall | 36 | 10 | 10 | 0% | 0.00 | 36 | Township |
| Glendo | 37 | 9 | 9 | 0% | 0.00 | 35 | Township |
| Sidney | 38 | 9 | 9 | 0% | 0.00 | 36 | Township |
| Vrooman | 39 | 6 | 6 | 0% | 0.00 | 36 | Township |
Lemmon is the largest city in Perkins County, with a population of 1,138.
Vrooman is the smallest city in Perkins County, with a population of 6.
There are 39 cities in Perkins County, South Dakota.