7 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Winchester | 1 | 27,353 | 28,013 | -0.48% | 2,975 | 9.19 | City |
| Shawneeland | 2 | 2,466 | 2,142 | 1.65% | 325 | 7.59 | CDP |
| Lake Holiday | 3 | 2,315 | 2,622 | 1.18% | 682 | 3.39 | CDP |
| Stephens City | 4 | 2,174 | 2,029 | 1.35% | 896 | 2.43 | Town |
| Middletown | 5 | 1,449 | 1,358 | 1.26% | 1,870 | 0.78 | Town |
| Brucetown | 6 | 294 | 213 | 7.3% | 519 | 0.57 | CDP |
| Gore | 7 | 45 | 113 | 7.14% | 51 | 0.88 | CDP |
Winchester is the largest city in Frederick County, with a population of 27,353.
Gore is the smallest city in Frederick County.
There are 7 cities in Frederick County, Virginia.