13 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Rose Hill | 1 | 21,447 | 21,846 | 0.38% | 3,818 | 6 | CDP |
| Pennington Gap | 2 | 1,566 | 1,612 | -0.57% | 939 | 2 | Town |
| Pennington Gap | 3 | 1,566 | 1,608 | -0.57% | 939 | 2 | Town |
| Jonesville | 4 | 847 | 869 | -0.47% | 762 | 1 | Town |
| Jonesville | 5 | 847 | 866 | -0.47% | 762 | 1 | Town |
| Big Stone Gap East | 6 | 820 | 709 | 7.19% | 1,712 | 0 | CDP |
| Dryden | 7 | 553 | 891 | -7.37% | 78 | 7 | CDP |
| Rose Hill | 8 | 535 | 567 | -3.25% | 156 | 3 | CDP |
| Ewing | 9 | 485 | 152 | 0.62% | 129 | 4 | CDP |
| Keokee | 10 | 305 | 459 | -2.24% | 65 | 5 | CDP |
| Stickleyville | 11 | 261 | 332 | 6.97% | 92 | 3 | CDP |
| Clinchport | 12 | 61 | 63 | -1.61% | 91 | 1 | Town |
| Clinchport | 13 | 61 | 63 | -1.61% | 91 | 1 | Town |
Rose Hill is the largest city in Lee County, with a population of 21,447.
Clinchport is the smallest city in Lee County, with a population of 61.
There are 13 cities in Lee County, Virginia.