7 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Charles Town | 1 | 8,436 | 6,526 | 4.74% | 1,438 | 6 | City |
| Ranson | 2 | 5,854 | 5,466 | 1.35% | 722 | 8 | Corporation |
| Shannondale | 3 | 3,278 | 3,364 | -0.15% | 389 | 8 | CDP |
| Shepherdstown | 4 | 1,541 | 1,531 | 0.13% | 3,843 | 0 | Town |
| Shenandoah Junction | 5 | 1,330 | 1,129 | 3.18% | 1,313 | 1 | CDP |
| Bolivar | 6 | 984 | 1,036 | -1.01% | 2,273 | 0 | Town |
| Harpers Ferry | 7 | 266 | 271 | -0.37% | 497 | 1 | Town |
Charles Town is the largest city in Jefferson County, with a population of 8,436.
Harpers Ferry is the smallest city in Jefferson County, with a population of 266.
There are 7 cities in Jefferson County, West Virginia.