9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Black River Falls | 1 | 3,638 | 3,510 | 0.72% | 869 | 4.18 | City |
| Merrillan | 2 | 534 | 564 | -1.11% | 421 | 1.27 | Village |
| Melrose | 3 | 522 | 544 | -0.76% | 652 | 0.80 | Village |
| Mission | 4 | 461 | 307 | 7.21% | 822 | 0.56 | CDP |
| Taylor | 5 | 458 | 482 | -1.08% | 628 | 0.73 | Village |
| Alma Center | 6 | 457 | 486 | -1.3% | 456 | 1 | Village |
| Hixton | 7 | 427 | 454 | -1.16% | 374 | 1.14 | Village |
| Sand Pillow | 8 | 306 | 278 | 6.99% | 1,313 | 0.23 | CDP |
| Millston | 9 | 142 | 108 | 0.71% | 136 | 1.04 | CDP |
Black River Falls is the largest city in Jackson County, with a population of 3,638.
Millston is the smallest city in Jackson County.
There are 9 cities in Jackson County, Wisconsin.