11 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| La Crosse | 1 | 50,429 | 52,673 | -0.88% | 2,323 | 21.71 | City |
| Onalaska | 2 | 19,118 | 18,868 | 0.26% | 1,802 | 10.61 | City |
| Holmen | 3 | 12,162 | 10,709 | 2.45% | 1,597 | 7.62 | Village |
| West Salem | 4 | 5,311 | 5,278 | 0.13% | 1,586 | 3.35 | Village |
| French Island | 5 | 4,281 | 4,318 | 0.12% | 2,118 | 2.02 | CDP |
| Brice Prairie | 6 | 1,959 | 2,200 | 0.21% | 427 | 4.59 | CDP |
| Bangor | 7 | 1,479 | 1,433 | 0.61% | 882 | 1.68 | Village |
| Rockland | 8 | 781 | 773 | 0.26% | 1,173 | 0.67 | Village |
| St. Joseph | 9 | 632 | 593 | 7.12% | 334 | 1.89 | CDP |
| Mindoro | 10 | 435 | 399 | 7.14% | 686 | 0.63 | CDP |
| Melvina | 11 | 93 | 92 | 0% | 191 | 0.49 | Village |
La Crosse is the largest city in La Crosse County, with a population of 50,429.
Melvina is the smallest city in La Crosse County.
There are 11 cities in La Crosse County, Wisconsin.