11 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Columbia | 1 | 131,345 | 126,307 | 0.77% | 1,951 | 67.31 | City |
| Ashland | 2 | 5,150 | 4,762 | 1.54% | 827 | 6.23 | City |
| Centralia | 3 | 4,839 | 4,561 | 1.17% | 1,662 | 2.91 | City |
| Hallsville | 4 | 1,808 | 1,617 | 2.15% | 1,129 | 1.60 | City |
| Sturgeon | 5 | 967 | 911 | 1.15% | 1,109 | 0.87 | City |
| Harrisburg | 6 | 297 | 269 | 2.06% | 373 | 0.80 | Town |
| Rocheport | 7 | 215 | 202 | 1.42% | 678 | 0.32 | City |
| Hartsburg | 8 | 141 | 131 | 1.44% | 656 | 0.21 | Town |
| Pierpont | 9 | 67 | 64 | 1.52% | 294 | 0.23 | Village |
| Huntsdale | 10 | 31 | 28 | 3.33% | 104 | 0.30 | Town |
| McBaine | 11 | 14 | 14 | 0% | 73 | 0.19 | Town |
Columbia is the largest city in Boone County, with a population of 131,345.
McBaine is the smallest city in Boone County.
There are 11 cities in Boone County, Missouri.