9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Dothan | 1 | 71,786 | 71,105 | 0.19% | 799 | 90 | City |
| Ashford | 2 | 2,432 | 2,255 | 1.46% | 392 | 6 | Town |
| Cowarts | 3 | 2,063 | 1,936 | 1.23% | 270 | 8 | Town |
| Webb | 4 | 1,342 | 1,275 | 0.98% | 119 | 11 | Town |
| Cottonwood | 5 | 1,102 | 1,055 | 0.82% | 191 | 6 | Town |
| Columbia | 6 | 710 | 694 | 0.42% | 181 | 4 | Town |
| Avon | 7 | 474 | 466 | 0.21% | 201 | 2 | Town |
| Gordon | 8 | 302 | 294 | 0.33% | 103 | 3 | Town |
| Madrid | 9 | 272 | 270 | 0% | 156 | 2 | Town |
Dothan is the largest city in Houston County, with a population of 71,786.
Madrid is the smallest city in Houston County, with a population of 272.
There are 9 cities in Houston County, Alabama.