9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Dothan | 1 | 71,377 | 71,079 | 0.08% | 794 | 90 | City |
| Ashford | 2 | 2,383 | 2,247 | 1.15% | 384 | 6 | Town |
| Cowarts | 3 | 2,042 | 1,936 | 1.04% | 267 | 8 | Town |
| Webb | 4 | 1,337 | 1,274 | 0.98% | 119 | 11 | Town |
| Cottonwood | 5 | 1,108 | 1,053 | 1% | 192 | 6 | Town |
| Columbia | 6 | 705 | 692 | 0.43% | 179 | 4 | Town |
| Avon | 7 | 477 | 466 | 0.42% | 202 | 2 | Town |
| Gordon | 8 | 299 | 294 | 0.34% | 102 | 3 | Town |
| Madrid | 9 | 260 | 264 | -0.38% | 149 | 2 | Town |
Dothan is the largest city in Houston County, with a population of 71,377.
Madrid is the smallest city in Houston County, with a population of 260.
There are 9 cities in Houston County, Alabama.