9 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Dothan | 1 | 71,922 | 71,105 | 0.19% | 796 | 90 | City |
| Ashford | 2 | 2,468 | 2,255 | 1.48% | 398 | 6 | Town |
| Cowarts | 3 | 2,089 | 1,936 | 1.26% | 273 | 8 | Town |
| Webb | 4 | 1,356 | 1,275 | 1.04% | 120 | 11 | Town |
| Cottonwood | 5 | 1,112 | 1,055 | 0.91% | 192 | 6 | Town |
| Columbia | 6 | 713 | 694 | 0.42% | 181 | 4 | Town |
| Avon | 7 | 476 | 466 | 0.42% | 201 | 2 | Town |
| Gordon | 8 | 304 | 294 | 0.66% | 104 | 3 | Town |
| Madrid | 9 | 273 | 270 | 0.37% | 156 | 2 | Town |
Dothan is the largest city in Houston County, with a population of 71,922.
Madrid is the smallest city in Houston County, with a population of 273.
There are 9 cities in Houston County, Alabama.