| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Madison | 1 | 67,388 | 57,310 | 2.56% | 2,205 | 31 | City |
| Athens | 2 | 36,014 | 25,733 | 5% | 860 | 42 | City |
| Triana | 3 | 5,901 | 3,005 | 8.91% | 2,107 | 3 | Town |
| Ardmore | 4 | 1,511 | 1,334 | 2.03% | 740 | 2 | Town |
| Elkmont | 5 | 459 | 414 | 1.77% | 279 | 2 | Town |
| Lester | 6 | 122 | 112 | 1.67% | 64 | 2 | Town |
| Mooresville | 7 | 48 | 44 | 2.13% | 192 | 0 | Town |
Madison is the largest city in Limestone County, with a population of 67,388.
Mooresville is the smallest city in Limestone County, with a population of 48.
There are 7 cities in Limestone County, Alabama.