| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Madison | 1 | 69,611 | 57,236 | 2.42% | 2,278 | 31 | City |
| Athens | 2 | 35,936 | 25,719 | 5.05% | 858 | 42 | City |
| Triana | 3 | 5,726 | 3,003 | 9.19% | 2,044 | 3 | Town |
| Ardmore | 4 | 1,516 | 1,332 | 2.09% | 742 | 2 | Town |
| Elkmont | 5 | 459 | 415 | 1.55% | 279 | 2 | Town |
| Lester | 6 | 124 | 111 | 1.64% | 65 | 2 | Town |
| Mooresville | 7 | 48 | 44 | 0% | 192 | 0 | Town |
Madison is the largest city in Limestone County, with a population of 69,611.
Mooresville is the smallest city in Limestone County, with a population of 48.
There are 7 cities in Limestone County, Alabama.