14 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Auburn | 1 | 85,429 | 76,585 | 2% | 1,367 | 62 | City |
| Auburn | 2 | 85,429 | 77,066 | 2% | 1,367 | 62 | City |
| Phenix City | 3 | 38,434 | 38,817 | -0.19% | 1,370 | 28 | City |
| Phenix City | 4 | 38,434 | 38,809 | -0.19% | 1,370 | 28 | City |
| Opelika | 5 | 35,988 | 31,005 | 2.81% | 588 | 61 | City |
| Opelika | 6 | 35,988 | 31,071 | 2.81% | 588 | 61 | City |
| Smiths Station | 7 | 5,620 | 5,436 | 0.66% | 687 | 8 | City |
| Smiths Station | 8 | 5,620 | 5,433 | 0.66% | 687 | 8 | City |
| Loachapoka | 9 | 163 | 162 | 0% | 143 | 1 | Town |
| Loachapoka | 10 | 163 | 161 | 0% | 143 | 1 | Town |
| Cusseta | 11 | 151 | 152 | 0% | 58 | 3 | Town |
| Cusseta | 12 | 151 | 151 | 0% | 58 | 3 | Town |
| Waverly | 13 | 147 | 149 | -0.68% | 53 | 3 | Town |
| Waverly | 14 | 147 | 149 | -0.68% | 53 | 3 | Town |
Auburn is the largest city in Lee County, with a population of 85,429.
Waverly is the smallest city in Lee County, with a population of 147.
There are 14 cities in Lee County, Alabama.