18 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Galena | 1 | 456 | 470 | -0.44% | 26 | 18 | City |
| Fort Yukon | 2 | 401 | 430 | -1.23% | 60 | 7 | City |
| Nenana | 3 | 331 | 355 | -1.19% | 53 | 6 | City |
| Huslia | 4 | 284 | 304 | -1.05% | 18 | 16 | City |
| McGrath | 5 | 278 | 296 | -1.07% | 6 | 43 | City |
| Tanana | 6 | 228 | 242 | -0.87% | 21 | 11 | City |
| Nulato | 7 | 220 | 234 | -0.9% | 5 | 41 | City |
| Grayling | 8 | 195 | 207 | -1.02% | 17 | 11 | City |
| Allakaket | 9 | 167 | 178 | -1.18% | 11 | 16 | City |
| Holy Cross | 10 | 167 | 178 | -1.18% | 6 | 29 | City |
| Kaltag | 11 | 150 | 161 | -1.32% | 6 | 23 | City |
| Ruby | 12 | 128 | 137 | -0.78% | 18 | 7 | City |
| Shageluk | 13 | 92 | 98 | -1.08% | 9 | 10 | City |
| Koyukuk | 14 | 90 | 96 | -1.1% | 16 | 6 | City |
| Nikolai | 15 | 82 | 81 | 0% | 19 | 4 | City |
| Hughes | 16 | 80 | 85 | -1.23% | 26 | 3 | City |
| Anvik | 17 | 70 | 73 | 0% | 7 | 9 | City |
| Bettles | 18 | 23 | 25 | 0% | 15 | 2 | City |
Galena is the largest city in Yukon-Koyukuk Census Area, with a population of 456.
Bettles is the smallest city in Yukon-Koyukuk Census Area, with a population of 23.
There are 18 cities in Yukon-Koyukuk Census Area, Alaska.