34 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Galena | 1 | 458 | 470 | -0.65% | 26 | 18 | City |
| Fort Yukon | 2 | 406 | 430 | -1.22% | 60 | 7 | City |
| Nenana | 3 | 335 | 355 | -1.18% | 56 | 6 | City |
| Huslia | 4 | 287 | 304 | -1.37% | 18 | 16 | City |
| McGrath | 5 | 281 | 296 | -1.06% | 7 | 42 | City |
| Tanana | 6 | 230 | 242 | -1.29% | 22 | 11 | City |
| Nulato | 7 | 222 | 234 | -1.33% | 5 | 41 | City |
| Arctic Village | 8 | 211 | 229 | 1.93% | 3 | 64 | CDP |
| Grayling | 9 | 197 | 207 | -1.01% | 18 | 11 | City |
| Allakaket | 10 | 169 | 178 | -1.17% | 11 | 15 | City |
| Holy Cross | 11 | 169 | 178 | -1.17% | 6 | 29 | City |
| Kaltag | 12 | 152 | 161 | -1.3% | 7 | 22 | City |
| Central | 13 | 131 | 38 | 7.38% | 1 | 248 | CDP |
| Ruby | 14 | 129 | 137 | -1.53% | 18 | 7 | City |
| Chalkyitsik | 15 | 119 | 118 | 7.21% | 14 | 9 | CDP |
| Beaver | 16 | 109 | 88 | 6.86% | 5 | 21 | CDP |
| Venetie | 17 | 95 | 126 | -5% | 4 | 23 | CDP |
| Shageluk | 18 | 93 | 98 | -1.06% | 9 | 10 | City |
| Koyukuk | 19 | 91 | 96 | -1.09% | 16 | 6 | City |
| Minto | 20 | 89 | 136 | -8.25% | 1 | 133 | CDP |
| Nikolai | 21 | 82 | 81 | 0% | 19 | 4 | City |
| Hughes | 22 | 81 | 85 | -1.22% | 27 | 3 | City |
| Takotna | 23 | 72 | 53 | 5.88% | 3 | 23 | CDP |
| Anvik | 24 | 70 | 73 | -1.41% | 7 | 9 | City |
| Rampart | 25 | 51 | 54 | 6.25% | 0.00 | 170 | CDP |
| Alatna | 26 | 45 | 30 | 7.14% | 1 | 36 | CDP |
| Evansville | 27 | 34 | 16 | 6.25% | 1 | 23 | CDP |
| Circle | 28 | 30 | 42 | -14.29% | 0.00 | 106 | CDP |
| Bettles | 29 | 23 | 25 | -4.17% | 15 | 2 | City |
| Manley Hot Springs | 30 | 21 | 42 | 5% | 0.00 | 55 | CDP |
| Lake Minchumina | 31 | 18 | 20 | 12.5% | 0.00 | 227 | CDP |
| Livengood | 32 | 16 | 7 | 6.67% | 0.00 | 265 | CDP |
| Stevens Village | 33 | 11 | 9 | 10% | 1 | 12 | CDP |
| Birch Creek | 34 | 4 | 4 | 0% | 0.00 | 8 | CDP |
Galena is the largest city in Yukon-Koyukuk Census Area, with a population of 458.
Birch Creek is the smallest city in Yukon-Koyukuk Census Area, with a population of 4.
There are 34 cities in Yukon-Koyukuk Census Area, Alaska.