Note: As defined by the US Census Bureau, a city's official boundaries often extend miles beyond the city itself and may even cross county lines. As such, several thousand US cities officially exist in two or more counties (and therefore appear on multiple counties' lists of cities.)
City | Rank | 2024 Pop. | 2020 Pop. | Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Galena | 1 | 453 | 469 | -0.88% | 26 | 17.7 | City |
| Fort Yukon | 2 | 405 | 428 | -1.46% | 60 | 6.7 | City |
| Nenana | 3 | 339 | 355 | -1.17% | 57 | 6.0 | City |
| Huslia | 4 | 289 | 304 | -1.36% | 18 | 15.8 | City |
| McGrath | 5 | 283 | 298 | -1.39% | 7 | 42.3 | City |
| Tanana | 6 | 232 | 243 | -1.28% | 22 | 10.7 | City |
| Nulato | 7 | 223 | 234 | -1.33% | 5 | 41.1 | City |
| Arctic Village | 8 | 207 | 229 | 1.97% | 3 | 63.8 | CDP |
| Grayling | 9 | 197 | 207 | -1.5% | 18 | 11.2 | City |
| Allakaket | 10 | 168 | 178 | -1.75% | 11 | 14.7 | City |
| Holy Cross | 11 | 167 | 177 | -1.76% | 6 | 28.8 | City |
| Kaltag | 12 | 155 | 163 | -1.27% | 7 | 21.6 | City |
| Ruby | 13 | 132 | 139 | -1.49% | 19 | 7.0 | City |
| Central | 14 | 122 | 38 | 7.02% | 0.00 | 247.9 | CDP |
| Chalkyitsik | 15 | 111 | 118 | 7.77% | 13 | 8.5 | CDP |
| Beaver | 16 | 102 | 88 | 8.51% | 5 | 20.5 | CDP |
| Venetie | 17 | 100 | 126 | -3.85% | 4 | 23.0 | CDP |
| Minto | 18 | 97 | 136 | -7.62% | 1 | 132.6 | CDP |
| Shageluk | 19 | 95 | 99 | -1.04% | 9 | 10.2 | City |
| Koyukuk | 20 | 93 | 97 | -1.06% | 16 | 5.7 | City |
| Nikolai | 21 | 85 | 84 | 0% | 19 | 4.4 | City |
| Hughes | 22 | 81 | 85 | -1.22% | 27 | 3.0 | City |
| Anvik | 23 | 70 | 73 | -1.41% | 7 | 9.5 | City |
| Takotna | 24 | 68 | 53 | 7.94% | 3 | 23.4 | CDP |
| Rampart | 25 | 48 | 54 | 9.09% | 0.00 | 169.8 | CDP |
| Alatna | 26 | 42 | 30 | 7.69% | 1 | 36.5 | CDP |
| Circle | 27 | 35 | 42 | -12.5% | 0.00 | 106.0 | CDP |
| Evansville | 28 | 32 | 16 | 10.35% | 1 | 23.0 | CDP |
| Bettles | 29 | 23 | 25 | -4.17% | 15 | 1.6 | City |
| Manley Hot Springs | 30 | 20 | 42 | 11.11% | 0.00 | 54.6 | CDP |
| Lake Minchumina | 31 | 16 | 20 | 6.67% | 0.00 | 227.0 | CDP |
| Livengood | 32 | 15 | 7 | 7.14% | 0.00 | 265.2 | CDP |
| Stevens Village | 33 | 10 | 9 | 0% | 1 | 11.7 | CDP |
| Birch Creek | 34 | 4 | 4 | 33.33% | 0.00 | 8.1 | CDP |
Note: City markers shown outside the county boundary mean that a part of the city extends into this county, but is primarily located in an adjacent county.
Galena is the largest city in Yukon-Koyukuk Census Area.
Birch Creek is the smallest city in Yukon-Koyukuk Census Area.
There are 34 cities in Yukon-Koyukuk Census Area, Alaska.