| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Galena | 1 | 447 | 466 | -0.67% | 25 | 18 | City |
| Fort Yukon | 2 | 400 | 429 | -1.23% | 60 | 7 | City |
| Nenana | 3 | 329 | 353 | -1.2% | 53 | 6 | City |
| Huslia | 4 | 284 | 303 | -1.05% | 18 | 16 | City |
| McGrath | 5 | 274 | 296 | -1.44% | 6.0 | 43 | City |
| Tanana | 6 | 231 | 247 | -1.28% | 22 | 11 | City |
| Nulato | 7 | 219 | 234 | -1.35% | 5.0 | 41 | City |
| Grayling | 8 | 195 | 208 | -1.02% | 17 | 11 | City |
| Allakaket | 9 | 166 | 178 | -1.19% | 10 | 16 | City |
| Holy Cross | 10 | 165 | 177 | -1.2% | 6.0 | 29 | City |
| Kaltag | 11 | 149 | 160 | -1.32% | 6.0 | 23 | City |
| Ruby | 12 | 129 | 138 | -1.53% | 18 | 7 | City |
| Shageluk | 13 | 92 | 98 | -1.08% | 9.0 | 10 | City |
| Koyukuk | 14 | 91 | 97 | -1.09% | 16 | 6 | City |
| Nikolai | 15 | 81 | 81 | 0% | 19 | 4 | City |
| Hughes | 16 | 80 | 85 | -1.23% | 26 | 3 | City |
| Anvik | 17 | 69 | 73 | -1.43% | 7.0 | 9 | City |
| Bettles | 18 | 22 | 24 | -4.35% | 14 | 2 | City |
Galena is the largest city in Yukon-Koyukuk Census Area, with a population of 447.
Bettles is the smallest city in Yukon-Koyukuk Census Area, with a population of 22.
There are 18 cities in Yukon-Koyukuk Census Area, Alaska.