9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Pine Bluff | 1 | 38,237 | 40,974 | -1.41% | 865 | 44 | City |
| White Hall | 2 | 5,478 | 5,583 | -0.38% | 775 | 7 | City |
| Redfield | 3 | 1,496 | 1,499 | -0.07% | 484 | 3 | City |
| Altheimer | 4 | 639 | 697 | -1.84% | 312 | 2 | City |
| Humphrey | 5 | 435 | 464 | -1.36% | 333 | 1 | City |
| Wabbaseka | 6 | 169 | 184 | -1.74% | 491 | 0 | Town |
| Tucker | 7 | 124 | 67 | 6.9% | 151 | 1 | CDP |
| Allport | 8 | 88 | 90 | -1.12% | 423 | 0 | Town |
| Sherrill | 9 | 50 | 55 | -1.96% | 379 | 0 | Town |
Pine Bluff is the largest city in Jefferson County, with a population of 38,237.
Sherrill is the smallest city in Jefferson County, with a population of 50.
There are 9 cities in Jefferson County, Arkansas.