8 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Midway | 1 | 21,148 | 19,311 | 1.61% | 1,760 | 12.01 | CDP |
| Quincy | 2 | 7,973 | 7,822 | 0.38% | 684 | 11.65 | City |
| Midway | 3 | 3,442 | 3,531 | -0.52% | 358 | 9.62 | City |
| Chattahoochee | 4 | 3,021 | 2,928 | 0.63% | 539 | 5.60 | City |
| Havana | 5 | 1,736 | 1,746 | -0.11% | 623 | 2.79 | Town |
| Midway | 6 | 1,363 | 1,417 | -1.59% | 1,849 | 0.74 | CDP |
| Gretna | 7 | 1,346 | 1,360 | -0.22% | 157 | 8.56 | City |
| Greensboro | 8 | 459 | 458 | 0% | 200 | 2.30 | Town |
Midway is the largest city in Gadsden County, with a population of 21,148.
Greensboro is the smallest city in Gadsden County.
There are 8 cities in Gadsden County, Florida.