| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Quincy | 1 | 8,226 | 7,892 | 0.78% | 706 | 12 | City |
| Midway | 2 | 3,470 | 3,544 | -0.34% | 361 | 10 | City |
| Chattahoochee | 3 | 3,035 | 2,931 | 0.8% | 542 | 6 | City |
| Havana | 4 | 1,762 | 1,744 | 0.17% | 632 | 3 | Town |
| Gretna | 5 | 1,297 | 1,279 | 0.08% | 152 | 9 | City |
| Greensboro | 6 | 470 | 458 | 0.43% | 205 | 2 | Town |
Quincy is the largest city in Gadsden County, with a population of 8,226.
Greensboro is the smallest city in Gadsden County, with a population of 470.
There are 6 cities in Gadsden County, Florida.