6 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Quincy | 1 | 8,230 | 7,896 | 0.69% | 706 | 12 | City |
| Midway | 2 | 3,459 | 3,537 | -0.37% | 360 | 10 | City |
| Chattahoochee | 3 | 3,078 | 2,927 | 0.82% | 550 | 6 | City |
| Havana | 4 | 1,760 | 1,748 | 0.11% | 632 | 3 | Town |
| Gretna | 5 | 1,284 | 1,283 | 0% | 150 | 9 | City |
| Greensboro | 6 | 467 | 458 | 0.43% | 203 | 2 | Town |
Quincy is the largest city in Gadsden County, with a population of 8,230.
Greensboro is the smallest city in Gadsden County, with a population of 467.
There are 6 cities in Gadsden County, Florida.