| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Lake City | 1 | 12,889 | 12,354 | 0.79% | 891 | 14 | City |
| Fort White | 2 | 674 | 621 | 1.35% | 263 | 3 | Town |
Lake City is the largest city in Columbia County, with a population of 12,889.
Fort White is the smallest city in Columbia County, with a population of 674.
There are 2 cities in Columbia County, Florida.