31 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Clermont | 1 | 52,145 | 43,335 | 3.5% | 2,894 | 18.02 | City |
| Leesburg | 2 | 34,794 | 27,111 | 4.62% | 884 | 39.34 | City |
| Groveland | 3 | 26,790 | 18,884 | 6.27% | 1,264 | 21.19 | City |
| Eustis | 4 | 25,281 | 23,221 | 1.66% | 2,190 | 11.54 | City |
| Tavares | 5 | 22,423 | 19,017 | 3.13% | 1,817 | 12.34 | City |
| Minneola | 6 | 20,531 | 14,205 | 6.57% | 1,844 | 11.14 | City |
| Mount Dora | 7 | 18,652 | 16,412 | 2.46% | 2,121 | 8.80 | City |
| Lady Lake | 8 | 17,593 | 16,027 | 1.81% | 1,884 | 9.34 | Town |
| Mascotte | 9 | 9,742 | 6,726 | 6.6% | 756 | 12.88 | City |
| Fruitland Park | 10 | 9,015 | 8,390 | 1.41% | 1,269 | 7.10 | City |
| Mount Plymouth | 11 | 4,995 | 4,812 | 1.34% | 1,736 | 2.88 | CDP |
| Umatilla | 12 | 3,984 | 3,746 | 1.22% | 1,091 | 3.65 | City |
| West DeLand | 13 | 3,584 | 3,271 | 0.11% | 1,667 | 2.15 | CDP |
| Lake Mack-Forest Hills | 14 | 2,880 | 2,425 | 4.54% | 595 | 4.84 | CDP |
| Silver Lake | 15 | 2,685 | 2,225 | 2.01% | 1,548 | 1.74 | CDP |
| Lake Kerr | 16 | 2,473 | 1,851 | 7.15% | 250 | 9.87 | CDP |
| Astatula | 17 | 2,155 | 1,899 | 2.42% | 687 | 3.13 | Town |
| Montverde | 18 | 1,830 | 1,662 | 1.89% | 988 | 1.85 | Town |
| Howey-in-the-Hills | 19 | 1,694 | 1,643 | 0.59% | 539 | 3.15 | Town |
| Astor | 20 | 1,447 | 1,242 | -0.48% | 619 | 2.34 | CDP |
| Yalaha | 21 | 1,414 | 1,547 | 0.21% | 249 | 5.67 | CDP |
| Pine Lakes | 22 | 1,377 | 1,127 | 2.53% | 860 | 1.60 | CDP |
| DeLand Southwest | 23 | 1,278 | 1,372 | 1.19% | 2,092 | 0.61 | CDP |
| North DeLand | 24 | 1,162 | 1,119 | -1.69% | 2,213 | 0.53 | CDP |
| Paisley | 25 | 1,134 | 1,301 | 1.89% | 362 | 3.13 | CDP |
| Lake Kathryn | 26 | 995 | 737 | 0.51% | 355 | 2.80 | CDP |
| Ferndale | 27 | 647 | 518 | 1.89% | 241 | 2.68 | CDP |
| Lisbon | 28 | 246 | 479 | -0.4% | 137 | 1.79 | CDP |
| Pittman | 29 | 223 | 249 | 1.36% | 177 | 1.26 | CDP |
| Okahumpka | 30 | 208 | 143 | -1.89% | 842 | 0.25 | CDP |
| Altoona | 31 | 32 | 77 | 6.67% | 71 | 0.45 | CDP |
Clermont is the largest city in Lake County, with a population of 52,145.
Altoona is the smallest city in Lake County.
There are 31 cities in Lake County, Florida.