23 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Clintonia | 1 | 7,235 | 7,315 | -0.1% | 241 | 30 | Township |
| Clinton | 2 | 6,854 | 6,963 | -0.32% | 1,934 | 4 | City |
| Santa Anna | 3 | 2,272 | 2,279 | 0.04% | 79 | 29 | Township |
| Farmer City | 4 | 1,805 | 1,819 | -0.17% | 754 | 2 | City |
| Clinton | 5 | 1,776 | 1,807 | -0.39% | 50 | 35 | Township |
| Texas | 6 | 1,248 | 1,255 | 0% | 35 | 36 | Township |
| Wapella | 7 | 899 | 914 | -0.33% | 31 | 29 | Township |
| Tunbridge | 8 | 737 | 726 | 0.55% | 20 | 37 | Township |
| McLean | 9 | 721 | 741 | -0.55% | 613 | 1 | Village |
| Waynesville | 10 | 634 | 638 | 0% | 26 | 24 | Township |
| Wapella | 11 | 502 | 512 | -0.4% | 911 | 1 | Village |
| Nixon | 12 | 487 | 489 | 0% | 18 | 28 | Township |
| Creek | 13 | 483 | 484 | 0% | 14 | 35 | Township |
| De Witt | 14 | 427 | 422 | 0.47% | 13 | 32 | Township |
| Waynesville | 15 | 378 | 382 | -0.26% | 1,185 | 0 | Village |
| Weldon | 16 | 368 | 370 | 0% | 1,378 | 0 | Village |
| Barnett | 17 | 365 | 366 | 0% | 10 | 37 | Township |
| Kenney | 18 | 310 | 304 | 0.32% | 1,099 | 0 | Village |
| Harp | 19 | 300 | 296 | 0.67% | 10 | 31 | Township |
| De Witt | 20 | 165 | 160 | 0.61% | 696 | 0 | Village |
| Lane | 21 | 161 | 164 | 7.33% | 1,309 | 0 | CDP |
| Rutledge | 22 | 151 | 152 | 0% | 6 | 24 | Township |
| Wilson | 23 | 127 | 125 | 0.79% | 5 | 24 | Township |
Clintonia is the largest city in De Witt County, with a population of 7,235.
Wilson is the smallest city in De Witt County, with a population of 127.
There are 23 cities in De Witt County, Illinois.