16 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Carbondale | 1 | 21,393 | 21,890 | -0.46% | 1,224 | 17.48 | City |
| Murphysboro | 2 | 6,883 | 7,082 | -0.58% | 1,312 | 5.25 | City |
| Crainville | 3 | 1,493 | 1,442 | 0.67% | 903 | 1.65 | Village |
| De Soto | 4 | 1,368 | 1,400 | -0.44% | 1,500 | 0.91 | Village |
| Harrison | 5 | 975 | 934 | 0% | 463 | 2.11 | CDP |
| Elkville | 6 | 812 | 836 | -0.61% | 1,059 | 0.77 | Village |
| Ava | 7 | 537 | 549 | -0.37% | 508 | 1.06 | City |
| Makanda | 8 | 525 | 537 | -0.38% | 99 | 5.28 | Village |
| Grand Tower | 9 | 463 | 477 | -0.64% | 371 | 1.25 | City |
| Dowell | 10 | 361 | 368 | -0.28% | 926 | 0.39 | Village |
| Blairsville | 11 | 350 | 322 | 7.03% | 347 | 1.01 | CDP |
| Campbell Hill | 12 | 299 | 311 | -0.66% | 647 | 0.46 | Village |
| Bush | 13 | 236 | 241 | -0.42% | 519 | 0.46 | Village |
| Vergennes | 14 | 231 | 235 | -0.43% | 656 | 0.35 | Village |
| Gorham | 15 | 165 | 170 | -0.6% | 135 | 1.22 | Village |
| St. Johns | 16 | 156 | 160 | -0.64% | 215 | 0.72 | Village |
Carbondale is the largest city in Jackson County, with a population of 21,393.
St. Johns is the smallest city in Jackson County.
There are 16 cities in Jackson County, Illinois.