31 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Carbondale | 1 | 24,012 | 24,644 | -0.62% | 642 | 37 | Township |
| Carbondale | 2 | 21,393 | 21,890 | -0.46% | 1,224 | 17 | City |
| Murphysboro | 3 | 9,169 | 9,452 | -0.71% | 253 | 36 | Township |
| Murphysboro | 4 | 6,883 | 7,082 | -0.58% | 1,312 | 5 | City |
| Makanda | 5 | 3,938 | 4,041 | -0.63% | 106 | 37 | Township |
| Somerset | 6 | 3,882 | 3,985 | -0.61% | 105 | 37 | Township |
| De Soto | 7 | 2,227 | 2,289 | -0.67% | 61 | 36 | Township |
| Elk | 8 | 1,696 | 1,741 | -0.64% | 47 | 36 | Township |
| Crainville | 9 | 1,493 | 1,442 | 0.67% | 903 | 2 | Village |
| De Soto | 10 | 1,368 | 1,400 | -0.44% | 1,500 | 1 | Village |
| Harrison | 11 | 975 | 934 | 0% | 463 | 2 | CDP |
| Levan | 12 | 817 | 839 | -0.61% | 24 | 33 | Township |
| Elkville | 13 | 812 | 836 | -0.61% | 1,059 | 1 | Village |
| Pomona | 14 | 761 | 782 | -0.65% | 15 | 50 | Township |
| Vergennes | 15 | 657 | 677 | -0.76% | 18 | 37 | Township |
| Sand Ridge | 16 | 656 | 676 | -0.76% | 19 | 35 | Township |
| Grand Tower | 17 | 542 | 561 | -0.91% | 17 | 32 | Township |
| Ava | 18 | 537 | 549 | -0.37% | 508 | 1 | City |
| Makanda | 19 | 525 | 537 | -0.38% | 99 | 5 | Village |
| Ora | 20 | 495 | 510 | -0.6% | 14 | 37 | Township |
| Grand Tower | 21 | 463 | 477 | -0.64% | 371 | 1 | City |
| Kinkaid | 22 | 444 | 456 | -0.67% | 12 | 36 | Township |
| Dowell | 23 | 361 | 368 | -0.28% | 926 | 0 | Village |
| Blairsville | 24 | 350 | 322 | 7.03% | 347 | 1 | CDP |
| Campbell Hill | 25 | 299 | 311 | -0.66% | 647 | 0 | Village |
| Bush | 26 | 236 | 241 | -0.42% | 519 | 0 | Village |
| Vergennes | 27 | 231 | 235 | -0.43% | 656 | 0 | Village |
| Fountain Bluff | 28 | 189 | 194 | -0.53% | 6 | 30 | Township |
| Gorham | 29 | 165 | 170 | -0.6% | 135 | 1 | Village |
| St. Johns | 30 | 156 | 160 | -0.64% | 215 | 1 | Village |
| Degognia | 31 | 140 | 144 | -0.71% | 5 | 30 | Township |
Carbondale is the largest city in Jackson County, with a population of 24,012.
Degognia is the smallest city in Jackson County, with a population of 140.
There are 31 cities in Jackson County, Illinois.