14 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Carbondale | 1 | 22,203 | 22,263 | -0.05% | 1,270 | 17 | City |
| Murphysboro | 2 | 7,027 | 7,078 | -0.11% | 1,339 | 5 | City |
| Crainville | 3 | 1,517 | 1,444 | 0.8% | 918 | 2 | Village |
| De Soto | 4 | 1,408 | 1,408 | 0% | 1,544 | 1 | Village |
| Elkville | 5 | 833 | 836 | 0% | 1,086 | 1 | Village |
| Ava | 6 | 546 | 549 | 0% | 517 | 1 | City |
| Makanda | 7 | 539 | 539 | 0% | 102 | 5 | Village |
| Grand Tower | 8 | 474 | 477 | 0% | 380 | 1 | City |
| Dowell | 9 | 367 | 366 | 0% | 941 | 0 | Village |
| Campbell Hill | 10 | 306 | 311 | -0.33% | 662 | 0 | Village |
| Vergennes | 11 | 237 | 234 | 0.42% | 673 | 0 | Village |
| Bush | 12 | 235 | 241 | -0.42% | 516 | 0 | Village |
| Gorham | 13 | 171 | 171 | 0% | 140 | 1 | Village |
| St. Johns | 14 | 154 | 162 | -0.65% | 212 | 1 | Village |
Carbondale is the largest city in Jackson County, with a population of 22,203.
St. Johns is the smallest city in Jackson County, with a population of 154.
There are 14 cities in Jackson County, Illinois.