8 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Columbus | 1 | 52,203 | 50,500 | 0.66% | 1,821 | 28.67 | City |
| Hope | 2 | 2,174 | 2,098 | 0.69% | 2,284 | 0.95 | Town |
| Taylorsville | 3 | 604 | 512 | -3.36% | 568 | 1.06 | CDP |
| Elizabethtown | 4 | 419 | 406 | 0.72% | 1,605 | 0.26 | Town |
| Grammer | 5 | 333 | 0 | 7.42% | 1,332 | 0.25 | CDP |
| Hartsville | 6 | 324 | 314 | 0.62% | 997 | 0.33 | Town |
| Clifford | 7 | 216 | 206 | 0.94% | 2,455 | 0.09 | Town |
| Jonesville | 8 | 181 | 176 | 0.56% | 1,248 | 0.14 | Town |
Columbus is the largest city in Bartholomew County, with a population of 52,203.
Jonesville is the smallest city in Bartholomew County.
There are 8 cities in Bartholomew County, Indiana.