| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Columbus | 1 | 53,067 | 50,538 | 0.78% | 1,767 | 30 | City |
| Hope | 2 | 2,197 | 2,098 | 0.73% | 2,308 | 1 | Town |
| Elizabethtown | 3 | 421 | 406 | 0.48% | 1,613 | 0 | Town |
| Hartsville | 4 | 328 | 314 | 0.61% | 1,009 | 0 | Town |
| Clifford | 5 | 213 | 204 | 0.47% | 2,420 | 0 | Town |
| Jonesville | 6 | 183 | 177 | 0.55% | 1,262 | 0 | Town |
Columbus is the largest city in Bartholomew County, with a population of 53,067.
Jonesville is the smallest city in Bartholomew County, with a population of 183.
There are 6 cities in Bartholomew County, Indiana.