6 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Columbus | 1 | 52,707 | 50,495 | 0.71% | 1,755 | 30 | City |
| Hope | 2 | 2,197 | 2,103 | 0.73% | 2,308 | 1 | Town |
| Elizabethtown | 3 | 422 | 406 | 0.72% | 1,617 | 0 | Town |
| Hartsville | 4 | 325 | 313 | 0.62% | 1,000 | 0 | Town |
| Clifford | 5 | 214 | 204 | 0.94% | 2,432 | 0 | Town |
| Jonesville | 6 | 181 | 175 | 0.56% | 1,248 | 0 | Town |
Columbus is the largest city in Bartholomew County, with a population of 52,707.
Jonesville is the smallest city in Bartholomew County, with a population of 181.
There are 6 cities in Bartholomew County, Indiana.