9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Osage | 1 | 3,471 | 3,635 | -0.94% | 1,548 | 2 | City |
| St. Ansgar | 2 | 1,153 | 1,170 | -0.26% | 1,059 | 1 | City |
| Stacyville | 3 | 424 | 456 | -1.4% | 823 | 1 | City |
| McIntire | 4 | 118 | 113 | 0.85% | 117 | 1 | City |
| Carpenter | 5 | 90 | 89 | 0% | 256 | 0 | City |
| Orchard | 6 | 67 | 66 | 0% | 663 | 0 | City |
| Little Cedar | 7 | 49 | 27 | 6.52% | 92 | 1 | CDP |
| Mona | 8 | 32 | 39 | 6.67% | 14 | 2 | CDP |
| Toeterville | 9 | 26 | 50 | 8.33% | 29 | 1 | CDP |
Osage is the largest city in Mitchell County, with a population of 3,471.
Toeterville is the smallest city in Mitchell County, with a population of 26.
There are 9 cities in Mitchell County, Iowa.