16 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Corydon | 1 | 1,573 | 1,522 | 0.64% | 1,151 | 1 | City |
| Corydon | 2 | 1,573 | 1,523 | 0.64% | 1,151 | 1 | City |
| Seymour | 3 | 648 | 635 | 0.31% | 271 | 2 | City |
| Seymour | 4 | 648 | 636 | 0.31% | 271 | 2 | City |
| Humeston | 5 | 473 | 464 | 0.21% | 758 | 1 | City |
| Humeston | 6 | 473 | 465 | 0.21% | 758 | 1 | City |
| Allerton | 7 | 433 | 427 | 0.23% | 365 | 1 | City |
| Allerton | 8 | 433 | 428 | 0.23% | 365 | 1 | City |
| Lineville | 9 | 194 | 192 | 0% | 215 | 1 | City |
| Lineville | 10 | 194 | 192 | 0% | 215 | 1 | City |
| Promise City | 11 | 91 | 89 | 0% | 495 | 0 | City |
| Promise City | 12 | 91 | 89 | 0% | 495 | 0 | City |
| Clio | 13 | 67 | 66 | 0% | 90 | 1 | City |
| Clio | 14 | 67 | 66 | 0% | 90 | 1 | City |
| Millerton | 15 | 37 | 36 | 0% | 127 | 0 | City |
| Millerton | 16 | 37 | 36 | 0% | 127 | 0 | City |
Corydon is the largest city in Wayne County, with a population of 1,573.
Millerton is the smallest city in Wayne County, with a population of 37.
There are 16 cities in Wayne County, Iowa.