3 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Nicholasville | 1 | 34,621 | 31,131 | 1.71% | 2,235 | 15 | City |
| Wilmore | 2 | 6,399 | 6,006 | 1.04% | 1,286 | 5 | City |
| Keene | 3 | 109 | 99 | 1.87% | 886 | 0 | City |
Nicholasville is the largest city in Jessamine County, with a population of 34,621.
Keene is the smallest city in Jessamine County, with a population of 109.
There are 3 cities in Jessamine County, Kentucky.