| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Nicholasville | 1 | 34,402 | 31,143 | 1.61% | 2,220 | 15 | City |
| Wilmore | 2 | 6,444 | 6,016 | 1% | 1,295 | 5 | City |
| Keene | 3 | 106 | 99 | 0.95% | 862 | 0 | City |
Nicholasville is the largest city in Jessamine County, with a population of 34,402.
Keene is the smallest city in Jessamine County, with a population of 106.
There are 3 cities in Jessamine County, Kentucky.