14 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Southaven | 1 | 58,154 | 54,645 | 1.15% | 1,407 | 41 | City |
| Southaven | 2 | 58,154 | 54,847 | 1.15% | 1,407 | 41 | City |
| Olive Branch | 3 | 47,565 | 46,395 | 0.42% | 852 | 56 | City |
| Olive Branch | 4 | 47,565 | 46,562 | 0.42% | 852 | 56 | City |
| Horn Lake | 5 | 26,532 | 26,733 | -0.16% | 1,656 | 16 | City |
| Horn Lake | 6 | 26,532 | 26,741 | -0.16% | 1,656 | 16 | City |
| Hernando | 7 | 18,858 | 17,152 | 1.7% | 742 | 25 | City |
| Hernando | 8 | 18,858 | 17,276 | 1.7% | 742 | 25 | City |
| Bridgetown | 9 | 3,181 | 2,580 | 3.11% | 1,467 | 2 | CDP |
| Lynchburg | 10 | 1,828 | 2,216 | -2.19% | 946 | 2 | CDP |
| Walls | 11 | 1,428 | 1,344 | 1.13% | 121 | 12 | Town |
| Walls | 12 | 1,428 | 1,348 | 1.13% | 121 | 12 | Town |
| Eudora | 13 | 598 | 485 | 7.17% | 152 | 4 | CDP |
| Lakeview | 14 | 492 | 324 | 6.96% | 2,424 | 0 | CDP |
Southaven is the largest city in DeSoto County, with a population of 58,154.
Lakeview is the smallest city in DeSoto County, with a population of 492.
There are 14 cities in DeSoto County, Mississippi.