9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Southaven | 1 | 58,189 | 54,843 | 1.16% | 1,408 | 41.33 | City |
| Olive Branch | 2 | 47,324 | 46,586 | 0.31% | 848 | 55.84 | City |
| Horn Lake | 3 | 26,285 | 26,742 | -0.34% | 1,640 | 16.02 | City |
| Hernando | 4 | 18,893 | 17,258 | 1.76% | 743 | 25.43 | City |
| Bridgetown | 5 | 3,181 | 2,580 | 3.11% | 1,467 | 2.17 | CDP |
| Lynchburg | 6 | 1,828 | 2,216 | -2.19% | 946 | 1.93 | CDP |
| Walls | 7 | 1,416 | 1,348 | 1% | 120 | 11.84 | Town |
| Eudora | 8 | 598 | 485 | 7.17% | 152 | 3.93 | CDP |
| Lakeview | 9 | 492 | 324 | 6.96% | 2,424 | 0.20 | CDP |
Southaven is the largest city in DeSoto County, with a population of 58,189.
Lakeview is the smallest city in DeSoto County.
There are 9 cities in DeSoto County, Mississippi.