24 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Cape Girardeau | 1 | 40,992 | 39,781 | 0.59% | 1,401 | 29 | City |
| Jackson | 2 | 15,939 | 15,446 | 0.63% | 1,483 | 11 | City |
| Jackson | 3 | 1,232 | 1,145 | 1.15% | 13 | 97 | Township |
| Jackson | 4 | 1,210 | 1,144 | 0.92% | 16 | 76 | Township |
| Jackson | 5 | 908 | 986 | -2.16% | 12 | 75 | Township |
| Jackson | 6 | 726 | 702 | 1.11% | 15 | 49 | Township |
| Gordonville | 7 | 627 | 622 | 0.16% | 804 | 1 | Village |
| Jackson | 8 | 554 | 537 | 0.91% | 8 | 70 | Township |
| Millersville | 9 | 387 | 363 | 6.91% | 342 | 1 | CDP |
| Delta | 10 | 375 | 375 | 0% | 952 | 0 | City |
| Altenburg | 11 | 337 | 344 | -0.3% | 321 | 1 | City |
| Dutchtown | 12 | 260 | 110 | 7% | 146 | 2 | CDP |
| Oak Ridge | 13 | 238 | 237 | 0% | 403 | 1 | Town |
| Shawneetown | 14 | 215 | 196 | 6.97% | 364 | 1 | CDP |
| Jackson | 15 | 186 | 189 | -0.53% | 3 | 56 | Township |
| Burfordville | 16 | 161 | 83 | 7.33% | 481 | 0 | CDP |
| Haywood City | 17 | 135 | 135 | 0% | 315 | 0 | Village |
| Jackson | 18 | 135 | 138 | -0.74% | 3 | 41 | Township |
| Jackson | 19 | 125 | 126 | -0.79% | 4 | 30 | Township |
| Allenville | 20 | 94 | 94 | 0% | 606 | 0 | Village |
| Pocahontas | 21 | 94 | 93 | 0% | 913 | 0 | Town |
| Whitewater | 22 | 85 | 85 | 0% | 397 | 0 | Town |
| Old Appleton | 23 | 71 | 71 | 0% | 587 | 0 | Town |
| New Wells | 24 | 41 | 41 | 7.89% | 70 | 1 | CDP |
Cape Girardeau is the largest city in Cape Girardeau County, with a population of 40,992.
New Wells is the smallest city in Cape Girardeau County, with a population of 41.
There are 24 cities in Cape Girardeau County, Missouri.