11 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Cape Girardeau | 1 | 41,334 | 39,786 | 0.63% | 1,412 | 29 | City |
| Jackson | 2 | 16,167 | 15,453 | 0.74% | 1,504 | 11 | City |
| Gordonville | 3 | 633 | 621 | 0.32% | 812 | 1 | Village |
| Delta | 4 | 381 | 375 | 0.26% | 967 | 0 | City |
| Altenburg | 5 | 334 | 342 | -0.3% | 318 | 1 | City |
| Oak Ridge | 6 | 239 | 235 | 0.42% | 404 | 1 | Town |
| Haywood City | 7 | 136 | 133 | 0.74% | 318 | 0 | Village |
| Pocahontas | 8 | 98 | 94 | 1.03% | 951 | 0 | Town |
| Allenville | 9 | 95 | 94 | 0% | 613 | 0 | Village |
| Whitewater | 10 | 85 | 84 | 0% | 397 | 0 | Town |
| Old Appleton | 11 | 75 | 74 | 0% | 620 | 0 | Town |
Cape Girardeau is the largest city in Cape Girardeau County, with a population of 41,334.
Old Appleton is the smallest city in Cape Girardeau County, with a population of 75.
There are 11 cities in Cape Girardeau County, Missouri.